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Description
Research Area
Images & Validation
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| Application Notes |
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Key Properties
−| Source | Escherichia Coli |
|---|---|
| Biological Activity | Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is < 0.3 ng/ml. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Protein Sequence | MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL |
Storage & Handling
−| Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
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| Form/Appearance | Sterile Filtered clear solution. |
| Buffer/Preservatives | TNF-b protein solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.1M NaCl and 1mM DTT. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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> 95% as determined by Bis-Tris PAGE
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98.00%
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500 μg, 100 μg, 1 mg

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Protocol Information
Human TNF b Protein (His Tag) (orb80621)
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