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Description
Research Area
,Product Categories/Proteins
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | Escherichia coli |
|---|---|
| Biological Activity | The ED50, as measured in a proliferation assay using HUVECs, is less than 8ng/ml. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Protein Sequence | MHHHHHHRSA PKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH |
Storage & Handling
−| Storage | Stability: Lyophilized TNFSF12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TWEAK should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Filtered White lyophilized (freeze-dried) powder. |
| Buffer/Preservatives | Lyophilized from a 0.2μm filtered solution in PBS. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−TWEAK, TNF-related weak inducer of apoptosis, TNFSF12, DR3LG, Apo3-Ligand, APO3L, TNFRSF12A, Tumor necrosis factor ligand superfamily member 12, MGC20669, MGC129581.
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Human TNFSF12 Protein, hFc Tag [orb757421]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 43.3 kDa after removal of the signal peptide.
Mammalian
50 μg, 10 μg, 100 μg 
Human sTWEAK ELISA Kit [orb339721]
Human
0.312 ng/ml-20 ng/ml
0.078 ng/ml
10 x 96 T, 48 T, 24 T, 5 x 96 T, 96 THuman TNFSF12 protein [orb358130]
Greater than 90% as determined by SDS-PAGE.
38.9 kDa
E.coli
20 μg, 100 μg, 1 mgRecombinantTWEAK,Human [orb1494661]
> 95% as analyzed by SDS-PAGE and HPLC.
20-22 kDa, observed by reducing SDS-PAGE.
CHO
50 μg, 10 μg, 1 mg
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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