Human TREM2 Protein, His Tag

SKU: orb735052

Description

Triggering receptor expressed on myeloid cells 2 (TREM2) is a cell surface receptor of the immunoglobulin superfamily. The TREM2 is found in various tissue macrophages, such as CNS microglia, bone osteoclasts, alveolar, peritoneal and intestinal macrophages. TREM2 is also present on cultured bone marrow-derived macrophages and monocyte-derived dendritic cells. Some research have identified a rare variant of TREM2 that is a risk factor for Alzheimer disease (AD), which is the most common form of late-onset dementia.The extracellular region of TREM2 contains a single immunoglobulin superfamily domain and binds polyanionic ligands, such as bacterial lipopolysaccharide (LPS) and phospholipids8. Upon ligand binding, TREM2 transmits intracellular signals through an adaptor, DAP12 (also known as TYRO protein tyrosine kinase-binding protein (TYROBP)), which is associated with the transmembrane region of TREM2 and which recruits the protein tyrosine kinase SYK through its cytosolic immunoreceptor tyrosine-based activation motifs (ITAMs). TREM2 is a pro-tumorigenic marker of tumor-infiltrating macrophages in mouse models and human tumors that can be targeted to curb tumor growth and improve the efficacy of checkpoint blockade therapy while remodeling the landscape of tumor-infiltrating macrophages.

Research Area

Immunology & Inflammation

Images & Validation

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Application Notes	This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.3 kDa. The protein migrates as 14 kDa, 35-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression System	HEK293
Biological Origin	Human
Target	TREM2
Reactivity	Human
Tag	C-10×His
Expression Region	His 19 - Ser 174
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated
MW	19.3 kDa
Purity	90%
Protein Sequence	NP_061838.1

Storage & Handling

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Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/Appearance	Powder
Buffer/Preservatives	PBS, pH 7.4
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Recombinant human TREM-2 Protein, His Tag [orb1551195]

Purity:	> 95% by SDS-PAGE.
Source:	Recombinant human TREM-2 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (His Tag19-Ser174) of human TREM-2 (Accession #NP_061838) fused with a 6��His Tag at the C-terminus.

Sizes Available: 100 μg, 50 μg

Recombinant Human Trem2 Protein, Avi His Tag [orb1550361]

Purity:	> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC
Source:	Recombinant Human Trem2 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (His Tag19-Ser174) of Human Trem2 fused with His Tag and Avi tag at the C-terminal.

Sizes Available: 100 μg

Biotinylated Recombinant Human Trem2 Protein, Avi His Tag [orb1550370]

Purity:	> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC
Source:	Biotinylated Recombinant Human Trem2 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (His Tag19-Ser174) of Human Trem2 fused with His Tag and Avi tag at the C-terminal.

Sizes Available: 100 μg

Human TREM2 Protein, His-Avi Tag (Biotin) [orb700636]
Conjugation:
Biotin
Purity:
90%
MW:
21.0 kDa
Sizes Available: 25 μg, 200 μg

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Quick Database Links

Gene Symbol

TREM2

UniProt

Q9NZC2

RefSeq (Protein):NP_061838.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_061838.1

Protocol Information

Protein Handling and Storage Guide

Protein Handling Guide

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Purity:	> 90%, determined by SDS-PAGE
Source:	This protein contains human TREM2 (NP_0618381) (Met 1-Ser 174) was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells.