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Description
Research Area
Product Categories/Products/Proteins,Product Categories/Research Area,Product Categories/Proteins,Epigenetics
Images & Validation
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| Tested Applications | ELISA, MS, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | E. coli |
|---|---|
| Target | UCHL3 |
| Reactivity | Human |
| Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
| Protein Sequence | MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA LEHHHHHH |
Storage & Handling
−| Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in a 0.2 μM filtered solution of 50mM Tris-HCl, 150mM NaCl, 1mM DTT, 50% Glycerol, pH 8.0 |
| Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 50mM Tris-HCl, 150mM NaCl, 1mM DTT, 50% Glycerol, pH 8.0 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Ubiquitin carboxyl-terminal hydrolase isozyme L3; UCH-L3; Ubiquitin thioesterase L3
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
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