Human VEGF121 Protein, His Tag

SKU: orb334936

Description

Human VEGF121, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 147 (Accession # P15692-9).

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 14.9 kDa. The protein migrates as 15 kDa,18-20 kDa under reducing (R) condition, and 33-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Immobilized Human VEGF121, His Tag at 1 µg/mL (100 µL/well) can bind Human VEGFR1/R2, Fc Tag with a linear range of 0.4-6 ng/mL (QC tested).
• Target: VEGF121
• Reactivity: Human
• Tag: N-6×His
• Expression Region: Ala 27 - Arg 147
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 14.9 kDa
• Purity: 95%
• Protein Sequence: Ala 27 - Arg 147

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of Human VEGF protein

Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations

Immobilized Human VEGF121, His Tag (Cat. No. orb334936) at 1 μg/mL (100 μL/well) can bind Human VEGFR1/R2, Fc Tag with a linear range of 0.4-6 ng/mL (QC tested).

Quick Database Links

Gene Symbol

VEGF121

UniProt

P15692