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Description
Research Area
Images & Validation
−| Application Notes |
|---|
Key Properties
−| Expression System | HEK293 |
|---|---|
| Biological Origin | Human |
| Biological Activity | Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165, premium grade with an affinity constant of 0.776 nM as determined in a SPR assay (Biacore T200) (Routinely tested). |
| Target | VEGF165 |
| Reactivity | Human |
| Tag | Native |
| Expression Region | Ala 27 - Arg 191 |
| Endotoxins | 0.01 EU per μg |
| Conjugation | Unconjugated |
| MW | 19.2 kDa |
| Purity | 95% |
| Protein Sequence | NP_001165097.1 |
Storage & Handling
−| Storage | -20°C to -70°C for 24 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
|---|---|
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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SDS-PAGE analysis of Human VEGF protein

Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations

Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations

Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165, premium grade (Cat. No. orb257923) with an affinity constant of 0.776 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_001165097.1 |
|---|
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Protocol Information
Human VEGF165 Protein (orb257923)
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