ICAD Antibody

SKU: orb248134

Description

During apoptosis, toxic or useless cells are broken down for removal. DFF, or DNA fragmentation factor, is a heterodimeric protein which mediates DNA fragmentation and chromatin condensation during apoptosis. DFFB is the active DFF subunit but remains inactive while bound to DFFA, also call ICAD (Inhibitor of caspase-activated DNase). Caspase-3 cleaves ICAD, which then disassociates from DFFB. The disassociation removes the inhibitory effects of ICAD, activating DFFB and beginning the DNA fragmentation process.

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot: 4-8ug/ml
• Reactivity: Mouse
• Application Notes:
  Provided assay concentrations are suggestions only, antibody titration may be required for optimal results.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A recombinant protein fragment from human ICAD was used as the immunogen for this antibody.
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot the antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This ICAD antibody is available for research use only.
• Disclaimer: For research use only

Quick Database Links

NCBI Gene ID

1676