IDE Antibody (Center)

SKU: orb1935700

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Primary Antibodies;Cardiovascular;Cell Biology;Metabolism;Neuroscience;Signal Transduction;Host cell receptor for virus entry;Viral entry into host cell;Host-virus interaction;SARS druggable targets;SARS CoV2 PPIs;AIDS Disease Progression;Infection

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:2000, WB: 1:1000, WB: 1:1000, WB: 1:2000, IHC-P: 1:10~50
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB12553
• Target: This IDE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-435 amino acids from the Central region of human IDE.
• Molecular Weight: 117968 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Insulin-degrading enzyme, Abeta-degrading protease, Insulin protease, Insulinase, Insulysin, IDE

Western blot analysis of anti-IDE Antibody (Center) in A375 cell line lysates (35 ug/lane). IDE (arrow) was detected using the purified Pab.

Western blot analysis of lysate from K562 cell line, using IDE Antibody (Center). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDE antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-IDE Antibody (Center) at 1:2000 dilution. Lane 1: A375 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 118 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-IDE Antibody (Center) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: A375 whole cell lysate. Lane 4: K562 whole cell lysate. Lane 5: mouse liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 118 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

This IDE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-435 amino acids from the Central region of human IDE.

UniProt

P14735

RefSeq (Protein):NP_004960.2, NP_001159418.1