IGF-BP1 Antibody (biotin)

SKU: orb1272631

Description

IGF-BP1 Antibody (biotin)

Research Area

,Other

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Application Notes:
  ELISA:Sandwich:To detect hIGF-BP1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human IGF-BP1 (38-265) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP1.Western Blot:To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

  Read more...

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Human IGF-BP1. Human IGF-BP1 specific antibody was purified by affinity chromatography and then biotinylated.
• Target: IGFBP1
• Conjugation: Unconjugated

Storage & Handling

• Storage: The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚°C. The reconstituted antibody is stable for at least two weeks at 2-80˚°C. Frozen aliquots are stable for at least 6 months when stored at -20˚°C. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AFBP, IBP1, PP12, IGF-BP25, hIGFBP-1, Insulin-like growth factor-binding protein 1, Placental protein 12, IBP-1

Quick Database Links

Gene Symbol

IGFBP1

UniProt

P08833

RefSeq:P08833