IGF-BP5 Antibody

SKU: orb1272619

Description

IGF-BP5 Antibody

Research Area

,Apoptosis,Cancer

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  ELISA:Sandwich:To detect hIGF-BP5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Polyclonal Biotinylated Anti-Human IGF-BP5 (38-154) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP5. Western Blot:: To detect hIGF-BP5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Immunohistochemistry:This antibody stained U-2 OS and U-251 MG cells. The primary antibody was incubated at 2.0 mg/mL overnight at 4˚C followed by a fluorescent labeled secondary antibody.

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Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: E.coli derived Recombinant Human IGF-BP5
• Target: IGFBP5
• Purification: IGF-BP5 specific antibody was purified by affinity chromatography employing immobilized hIGF-BP5 matrix.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IBP5, IBP5, Insulin-like growth factor-binding protein 5IBP-5

Quick Database Links

Gene Symbol

IGFBP5

UniProt

P24593

RefSeq:P24593