IGF1 Receptor (IGF1R) Antibody (C-term)

SKU: orb1428633

Description

Rabbit polyclonal antibody to IGF1 Receptor (IGF1R).

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Epigenetics,Primary Antibodies;Cancer;Developmental Biology;Neuroscience;Signal Transduction;Anti-Kinome Antibodies

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:2000, WB: 1:2000, WB: 1:1000, IHC-P: 1:50~100
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB01458
• Immunogen: Synthetic Peptide
• Target: IGF1R
• Molecular Weight: 154793
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, IGF-I receptor, CD221, Insulin-like growth factor 1 receptor alpha chain, Insulin-like growth factor 1 receptor beta chain, IGF1R

All lanes: Anti-IGF1 Receptor (IGF1R) Antibody (C-term) at 1:2000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: C2C12 whole cell lysate. Lane 4: C6 whole cell lysate. Lane 5: Hela whole cell lysate. Lane 6: NIH/3T3 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 155 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-IGF1 Receptor (IGF1R) Antibody (C-term) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Hela whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 155 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of anti-IGF1R Pab in SK-BR-3 cell lysate. IGF1R (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Quick Database Links

Gene Symbol

IGF1R

UniProt

P08069

RefSeq (Protein):NP_000866.1