IL-12 Antibody (biotin)

SKU: orb1671445

Description

IL-12 Antibody (biotin)

Research Area

,COVID-19,Immunology,Cancer,Stem Cell,Other

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Mouse
• Application Notes:
  Sandwich ELISA: To detect mIL-12 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Murine IL-12 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mIL-12. Western Blot To detect mIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

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Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mIL-12. Anti-Murine IL-12 specific antibody was purified by affinity chromatography and then biotinylated.
• Target: Il12a , Il12b
• Purification: IL-12 specific antibody was purified by affinity chromatography and then biotinylated
• Conjugation: Unconjugated

Storage & Handling

• Storage: Antibody is stable for at least 2 years from date of receipt at -20˚°C. The reconstituted antibody is stable for at least two weeks at 2-8˚°C. Frozen aliquots are stable for at least 6 months when stored at -20˚°C. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Interleukin-12, NKSF, CTL Maturation Factor (TCMF), Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF p35: Interleukin-12 subunit alpha, IL-12 subunit p35, IL-12A, Cytotoxic Lymphocyte Maturation Factor 35 kDa subunit (CLMF p35), NK cell Stimulating Factor Chain 1 p40: Interleukin-12 subunit beta, IL-12 subunit p40, IL-12B, CytotoxicLymphocyte Maturation Factor 40 kDa subunit (CLMF p40), NK cell Stimulating Factor Chain 2

To detect mIL-12 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine IL-12 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mIL-12.

To detect mIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Quick Database Links

Gene Symbol

Il12a , Il12b

UniProt

P43432, P43431

RefSeq:p35, P43431, P43432, p40