IL-1a Antibody

SKU: orb1272473

Description

IL-1a Antibody

Research Area

,Immunology,Chemokines & Cytokines

Images & Validation

• Tested Applications: ELISA, NeA, WB
• Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1-alpha (murine IL-1 alpha).
• Target: Il1a
• Purification: Anti-mIL-1 alpha specific antibody was purified by affinity chromatography employing immobilized mIL-1 alpha matrix.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Il-1aInterleukin-1 alphaIL-1 alpha

To detect mIL-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Murine IL-1-alpha (orb1272472) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1-alpha.

To detect mIL-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect mIL-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained colchicine injected mouse brain (including the hippocampal fissure) tissue. The primary antibody was incubated at 1.0 mg/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.

This antibody stained colchicine injected mouse brain (including the hippocampal fissure) tissue. The primary antibody was incubated at 1.0 mg/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.

Quick Database Links

Gene Symbol

Il1a

UniProt

P01582

RefSeq:P01582