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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Target | IL21 |
| Tag | Fc Tag |
| MW | 41.9 kDa |
| Purity | > 95% as determined by SDS-PAGE. |
| Protein Sequence | Gln 30 - Ser 162 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH 7.5 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human IL21 protein (Active) [orb358984]
> 97% as determined by SDS-PAGE and HPLC.
15.4 kDa
E.Coli
10 μg, 100 μg, 500 μgMouse IL21 protein (Active) [orb359019]
> 98% as determined by SDS-PAGE and HPLC.
15.0 kDa
E.Coli
10 μg, 100 μg, 500 μgHuman IL21 protein [orb605530]
Greater than 85% as determined by SDS-PAGE.
16.9 kDa
Yeast
1 mg, 20 μg, 100 μgRecombinant human IL-21 protein (HEK293) [orb1516417]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 38-55 kDa based on Tris-Bis PAGE result. kDa
100 μg, 50 μg

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Human IL-21, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Immobilized Human IL-21 R, His Tag at 2 ug/ml (100 uL/well) can bind Human IL-21, Fc Tag with a linear range of 0.2-8 ng/mL.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_068575.1 |
|---|
Documents Download
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Protocol Information
IL-21 Recombinant Protein (orb1231259)
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