IL-23 Rabbit Polyclonal Antibody

SKU: orb10907

Description

IL-23 Rabbit Polyclonal Antibody

Research Area

Interleukins, Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IL-23
• Target: Il23a
• Molecular Weight: 21 kDa
• Purification: affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Interleukin-23 subunit alpha; Il23a; IL 23; IL 23A; IL23P19; Interleukin 23 alpha subunit p19; JKA3 induced upon T cell activation; P19; SGRF; IL-23 subunit alpha; IL-23-A; IL-23p19; IL23; Interleukin 23 p19 subunit; Interleukin-23 subunit p19; JKA3 induced upon T cell activation; MGC79388; IL23A_HUMAN.

Sample: Lane 1: Mouse Ear tissue lysates, Lane 2: Human A431 cell lysates, Lane 3: Human Jurkat cell lysates, Primary: Anti-IL-23 (orb10907) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kDa, Observed band size: 21 kDa.

Tissue/Cell: human rhinopolypus tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IL-23 Polyclonal Antibody, Unconjugated (orb10907) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

Il23a

UniProt

Q9NPF7

A Wojtkowiak-Giera 1, D Kosik-Bogacka 2, N Łanocha-Arendarczyk 3, A Kolasa 4, K Kot 2, P Solarczyk 1, M Derda Toll-like receptors and inflammatory cytokines in the skin of Acanthamoeba spp. infected immunocompetent and immunosuppressed mice Exp Parasitol ., (2025)

PubMed: 40274043

Applications

IHC

Reactivity

Mouse