IL-4 Antibody (biotin)

SKU: orb1272451

Description

IL-4 Antibody (biotin)

Research Area

,Immunology,Chemokines & Cytokines

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Rat
• Application Notes:
  ELISA:Sandwich:To detect Rat IL-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat IL-4 (XP-5190) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-4.Western Blot:To detect rat IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

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Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat IL-4 (rat Interleukin-4).
• Target: Il4
• Purification: Anti-rat IL-4 specific antibody was purified by affinity chromatography and then biotinylated.
• Conjugation: Unconjugated

Storage & Handling

• Storage: IL-4 antibody is stable for at least 2 years from date of receipt at -20˚°C. The reconstituted antibody is stable for at least two weeks at 2-8˚°C. Frozen aliquots are stable for at least 6 months when stored at -20˚°C. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Il4e12, Il-4, Interleukin-4, B-cell IGG differentiation factor, IL-4

Quick Database Links

Gene Symbol

Il4

UniProt

P20096

RefSeq:P20096