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Description
Research Area
,Cancer,Chemokines & Cytokines,Immunology,Obesity,Signal Transduction,Other
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Target | IL5 |
| Tag | His Tag |
| MW | 15.2 kDa |
| Purity | > 90% as determined by SDS-PAGE. |
| Protein Sequence | Met 20 - Ser 134 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−IL-5,TRF,IL5,Interleukin-5
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Human IL5 Protein, His Tag [orb689428]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 13.94 kDa after removal of the signal peptide.The apparent molecular mass of IL5-His is approximately 15-25 kDa due to glycosylation.
Mammalian
50 μg, 100 μg, 10 μg
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Rabbit IL-5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Rabbit IL-5, His Tag at 5 ug/ml (100 uL/well) can bind Human IL-5 R alpha, Fc Tag with a linear range of 2-78 ng/mL.
Quick Database Links
Gene Symbol
IL5
RefSeq:XP_002710247.1
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| RefSeq | XP_002710247.1 |
|---|
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL-5 Recombinant Protein (orb1231529)
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