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Description
Research Area
,Cancer,Chemokines & Cytokines,Immunology,Neuroscience,Obesity,Signal Transduction
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Recombinant IL-6 is fully biologically active when compared to standards.ED50 is less than 0.1 ng/ml as determined by the dose-dependent stimulation of human TF-1 cells.Specific Activity of 5.0 x 107 IU/mg. |
| Target | IL6 |
| Tag | Tag Free |
| MW | 20.9 kD |
| Purity | Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
| Protein Sequence | Pro29-Met212 |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20˚°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7˚°C for 2-7 days.Aliquots of reconstituted samples are stable at -20˚°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 5.0. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-6, IL-6, B-Cell Stimulatory Factor 2, BSF-2, CTL Differentiation Factor, CDF, Hybridoma Growth Factor, Interferon Beta-2, IFN-Beta-2, IL6, IFNB2
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
IL-6 Recombinant Protein (orb1229854)
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