IL1 beta Antibody / IL1B

SKU: orb1823862

Description

Recognizes a protein of 17-31kDa, identified as interleukin 1beta. It shows no cross-reaction with interleukin 1alpha. Epitopes of mAb IL1B/463 and IL1B/4650 are different and provide a good pair for developing an ELISA. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Images & Validation

• Tested Applications: ELISA, IHC-P
• Dilution Range: ELISA (Order BSA-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: IL1B/4650
• Immunogen: A recombinant partial protein sequence (within amino acids 100-269) from the human protein was used as the immunogen for the Interleukin-1 beta antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot the Interleukin-1 beta antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This Interleukin-1 beta antibody is available for research use only.
• Disclaimer: For research use only

Quick Database Links

UniProt

P01584