IL17RA Antibody

SKU: orb1238913

Description

IL17RA Antibody

Research Area

,Immunology

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1-4 μg/mL; IHC-P/IF: 10-20 μg/mL. Antibody validated: Western Blot in human and mouse samples; Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-IL-17RA antibody was raised against a peptide corresponding to 18 amino acid synthetic peptide near the amino terminus of human IL-17RA. The immunogen is located within amino acids 150-200 of IL-17RA.
• Target: IL17RA
• Molecular Weight: Predicted: 96 kD Observed: 100 kD
• Purification: IL-17RA Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: IL-17RA Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IL-17RA Antibody: CD217, IL17R, CANDF5, CDw217, IL-17RA, hIL-17R, Interleukin-17 receptor A, IL-17 receptor A

WB Validation in K562 Cells. Loading: 10 μg of lysate. Antibodies: IL-17RA, orb1238913, 4 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Mouse Tissues. Loading: 15 μg of lysate. Antibodies: IL-17RA, orb1238913, 2 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of IL-17RA in Human Spleen. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling IL-17RA with orb1238913 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Immunofluorescence Validation of IL-17RA in Mouse Thymus. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus tissue labeling IL-17RA with orb1238913 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Quick Database Links

Gene Symbol

IL17RA

UniProt

Q96F46

RefSeq (Protein):NP_055154