IMPDH2 monoclonal antibody (M01), clone 1E12-B6

SKU: orb2292716

Description

Mouse monoclonal antibody raised against a full length recombinant IMPDH2.

Images & Validation

• Tested Applications: ELISA, IP, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 kappa
• Clone No.: 1E12-B6
• Immunogen: IMPDH2 (AAH12840, 1 a.a. ~ 514 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MADYLISGGTSYVPDDGLTAQQLFNCGDGLTYNDFLILPGYIDFTADQVDLTSALTKKITLKTPLVSSPMDTVTEAGMAIAMALTGGIGFIHHNCTPEFQANEVRKVKKYEQGFITDPVVLSPKDRVRDVFEAKARHGFCGIPITDTGRMGSRLVGIISSRDIDFLKEEEHDCFLEEIMTKREDLVVAPAGITLKEANEILQRSKKGKLPIVNEDDELVAIIARTDLKKNRDYPLASKDAKKQLLCGAAIGTHEDDKYRLDLLAQAGVDVVVLDSSQGNSIFQINMIKYIKDKYPNLQVIGGNVVTAAQAKNLIDAGVDALRVGMGSGSICITQEVLACGRPQATAVYKVSEYARRFGVPVIADGGIQNVGHIAKALALGASTVMMGSLLAATTEAPGEYFFSDGIRLKKYRGMGSLDAMDKHLSSQNRYFSEADKIKVAQGVSGAVQDKGSIHKFVPYLIAGIQHSCQDIGAKSLTQVRAMMYSGELKFEKRTSSAQVEGGVHSLHSYEKRLF
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Detection limit for recombinant GST tagged IMPDH2 is approximately 0.3 ng/ml as a capture antibody.

Immunoprecipitation of IMPDH2 transfected lysate using anti-IMPDH2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IMPDH2 MaxPab rabbit polyclonal antibody.

Western Blot analysis of IMPDH2 expression in transfected 293T cell line by IMPDH2 monoclonal antibody (M01), clone 1E12-B6. Lane 1: IMPDH2 transfected lysate (55.8 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of IMPDH2 over-expressed 293 cell line, cotransfected with IMPDH2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with IMPDH2 monoclonal antibody (M01), clone 1E12-B6 (Cat # orb2292716). GAPDH (36.1 kDa) used as specificity and loading control.

Quick Database Links

RefSeq:AAH12840