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Description
Research Area
Images & Validation
−| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:1000, IHC-P-Leica: 1:500 |
| Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB01419 |
| Target | This INSR(Insulin Receptor) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-57 amino acids from the N-terminal region of human INSR(Insulin Receptor). |
| Molecular Weight | 156333 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−INSR(Insulin Receptor) Antibody (N-term) [orb527256]
IHC-P, WB
Mouse
Rabbit
Polyclonal
Unconjugated
50 μlINSR(Insulin Receptor) Antibody (N-term) [orb1166311]
IHC-P, WB
Human, Mouse
Rabbit
Polyclonal
Unconjugated
100 μl, 30 μl

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Anti-INSR (Insulin Receptor) Antibody (N-term) at 1:1000 dilution + Mouse liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 156 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_001073285.1, NP_000199.2 |
|---|
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Protocol Information
INSR(Insulin Receptor) Antibody (N-term) (orb1929020)
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