Interferon gamma Antibody / IFNG

SKU: orb2639889

Description

Recognizes a protein of 20-25kDa, identified as human interferon. This mAb is specific to human IFNg and recognizes both recombinant and native human IFNg. T lymphocytes and NK cells mainly produce IFNg. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses, including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak anti-viral and anti-proliferative activity, and potentiates the antiviral and anti-tumor effects of IFNa/b (type I interferon).

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ACTH antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: IFNG/466
• Immunogen: Human recombinant protein was used as the immunogen for the IFN gamma antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This IFN gamma antibody is available for research use only.
• Disclaimer: For research use only

IHC testing of FFPE human renal cell carcinoma with IFN gamma antibody (clone IFNG/466). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 10-20 min.

IHC testing of FFPE human renal cell carcinoma with IFN gamma antibody (clone IFNG/466). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 10-20 min.

IHC testing of FFPE human testis with IFN gamma antibody (clone IFNG/466). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free IFN gamma Antibody (clone IFNG/466). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P01579