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Interferon Gamma Antibody

SKU: orb345094

Description

IFN gamma antibody

Research Area

Product Categories/Products/Antibodies,Immunogen Type: Recombinant Protein

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:20,000, WB: 1:500-1:2,000
ReactivityGallus
Application Notes
This protein A purified IFN-gamma antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 16.7 kDa in size corresponding to chicken IFN gamma by western blotting in the appropriate cell lysate or extract.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenThis protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to 145 amino acids of mature chicken IFN gamma.
PurityThis product was protein A purified from monospecific antiserum by chromatography. This antibody is specific for chicken IFN gamma protein. A BLAST analysis was used to suggest cross-reactivity with IFN gamma from chicken based on 100% homology; ring-necked pheasant, turkey, quail, and junglefowl based on 91-97% homology with the immunizing sequence. Cross-reactivity with IFN gamma from other sources has not been determined.
ConjugationUnconjugated

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/Preservatives0.01% (w/v) Sodium Azide
Concentration1.0 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

rabbit anti-Interferon gamma, IFNG, rabbit anti-IFN gamma

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 730.00
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Free Secondary Antibody (20 ul)0/0

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