ISG15 Antibody (N-term))

SKU: orb349720

Description

Mouse monoclonal antibody to ISG15.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Immunology,Epigenetics,Infectious Diseases,Crown Antibodies;Primary Antibodies;Cell Biology;Antiviral defense;Host-virus interaction;Immunity;Innate immunity;RIG-l-like Receptor

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:8000
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: 1031CT2.8.3
• Immunogen: Recombinant Protein
• Target: ISG15
• Molecular Weight: 17888
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-G1P2 antibody, anti-hUCRP antibody, anti-IFI 15 antibody, anti-IFI15 antibody, anti-Interferon induced protein 15 antibody, anti-Interferon induced protein IFI-15K antibody, anti-Interferon-induced 17-kDa/15-kDa protein antibody, anti-IP17 antibody, anti-ISG 15 antibody, anti-ISG15 antibody, anti-ISG-15 antibody, anti-ISG15 ubiquitin like modifier antibody, anti-Ubiquitin cross-reactive protein antibody, anti-Ubiquitin-like protein ISG15 antibody, anti-UCRP antibody

All lanes: Anti-ISG15 Antibody (N-term) at 1:8000 dilution. Lane 1: Hela whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 18 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling ISG15 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

Quick Database Links

Gene Symbol

ISG15

UniProt

P05161