ITGA2 Antibody, KO Validated

SKU: orb1274426

Description

ITGA2 Antibody, KO Validated

Research Area

,Cancer,Cell Cycle,Immunology,Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant protein of human ITGA2
• Target: ITGA2
• Molecular Weight: Observed: 150kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ITGA2, Alpha2 integrin, BR, CD49b antigen, CD49B, Integrin alpha2, Integrin Alpha 2, Integrin alpha-2, HPA-5, VLA-2, VLAA2, VLA-2 subunit alpha, Collagen receptor, GPIa, Platelet antigen Br, Platelet glycoprotein GPIa, VLA2 receptor, alpha-2 subunit

Western blot analysis of extracts of various cell lines, using ITGA2 antibody (orb1274426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Western blot analysis of extracts from normal (control) and ITGA2 knockout (KO) HeLa cells, using ITGA2 antibody (orb1274426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat brain using ITGA2 Antibody (orb1274426) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using ITGA2 Antibody (orb1274426) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using ITGA2 antibody (orb1274426) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

ITGA2

UniProt

P17301