ITGAX Antibody

SKU: orb1259678

Description

ITGAX Antibody

Research Area

,Cell Cycle,Immunology,Signal Transduction,Stem Cell

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-350 of human ITGAX (NP_000878.2).
• Target: ITGAX
• Molecular Weight: Observed: 150kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Integrin alpha-X, CD11 antigen-like family member C, Leu M5, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor p150, CD11c, ITGAX, CD11C

Western blot analysis of extracts of various cell lines, using ITGAX antibody (orb1259678) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded rat lung using ITGAX antibody (orb1259678) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using ITGAX antibody (orb1259678) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using ITGAX antibody (orb1259678) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using ITGAX antibody (orb1259678) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

ITGAX

UniProt

P20702