JNK1/2/3 (phospho Tyr185) rabbit pAb

SKU: orb764374

Description

Rabbit polyclonal antibody to JNK1/2/3 (phospho-Tyr185)

Research Area

Product Categories/Antibodies/Primary Antibodies,Product Categories/Small Molecules,Product Categories/Antibodies

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution Range: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human SAPK/JNK around the phosphorylation site of Tyr185. AA range:151-200
• Molecular Weight: 45
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-MAPK8 antibody, anti-JNK1 antibody, anti-PRKM8 antibody, anti-SAPK1 antibody, anti-SAPK1C antibody, anti-Mitogen-activated protein kinase 8 antibody, anti-MAP kinase 8 antibody, anti-MAPK 8 antibody, anti-JNK-46 antibody, anti-Stress-activated protein kinase 1c antibody, anti-SAPK1c antibody, anti-Stress-activated protein kinase JNK1 antibody, anti-c-Jun N-terminal antibody

Immunofluorescence analysis of human-lung tissue. 1, JNK1/2/3 (phospho Tyr185) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Immunofluorescence analysis of human-lung tissue. 1, JNK1/2/3 (phospho Tyr185) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Immunofluorescence analysis of human-stomach tissue. 1, JNK1/2/3 (phospho Tyr185) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Immunofluorescence analysis of human-stomach tissue. 1, JNK1/2/3 (phospho Tyr185) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.