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Description
Research Area
,Apoptosis,Cancer,Growth Factors,Immunology
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Potent biologically active trimer. Induces apoptosis in a concentration range of 10-100ng/ml. Note: Does not require a cross-linking enhancer for its potent biological activity. ED50: 20ng/ml (BJAB cells). For cell lines that require extensive cross-linking of the TRAIL-Rs for killing (e.g. Jurkat) use SuperKillerTRAIL (Prod. No. AG-40T-0002). |
| Target | TNFSF10 |
| Tag | His Tag |
| Purity | > 98% (SDS-PAGE). Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza). |
| Protein Sequence | The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. |
Storage & Handling
−| Storage | Stable for at least 1 year after receipt when stored at -80˚°C. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | In 20mM HEPES, pH 7.4, 150mM NaCl, 0.005% Tween 20, 1% sucrose, 1mM DTT. |
| Concentration | 0.5 mg/ml |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−TRAIL, Apo-2L, TNFSF10, CD253
Quality Guarantee
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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