KIT Antibody (N-term D121)

SKU: orb38330

Description

Rabbit polyclonal antibody against c-Kit

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Primary Antibodies;Cancer;Developmental Biology;Immunology;Neuroscience;Signal Transduction;Anti-Kinome Antibodies;Immune system process

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:1000, FACS: 1:10-50, IHC-P: 1:10-50
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB15297
• Immunogen: Synthetic Peptide
• Target: KIT
• Molecular Weight: 109865
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-C Kit antibody, anti-c-Kit antibody, anti-CD 117 antibody, anti-CD117 antibody, anti-CD117 antigen antibody, anti-KIT antibody, anti-KIT oncogene antibody, anti-Mast cell growth factor receptor antibody, anti-Mast/stem cell growth factor receptor antibody, anti-Mast/stem cell growth factor receptor Kit antibody, anti-p145 c-Kit antibody, anti-PBT antibody, anti-Piebald trait protein antibody, anti-Proto oncogene c Kit antibody, anti-Proto oncogene tyrosine protein kinase Kit antibody, anti-Proto-oncogene c-Kit antibody, anti-SCF Receptor antibody, anti-SCFR antibody, anti-soluble KIT variant 1 antibody, anti-Steel Factor Receptor antibody, anti-Stem cell factor receptor antibody, anti-tyrosine protein kinase Kit antibody, anti-Tyrosine-protein kinase Kit antibody

Anti-KIT Antibody (N-term D121) at 1:1000 dilution + Hela whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 110 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Flow cytometric analysis of NCI-H460 cells using KIT Antibody (N-term D121) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

KIT Antibody (N-term D121) western blot analysis in mouse testis tissue lysates (35 ug/lane). This demonstrates the KIT antibody detected the KIT protein (arrow).

KIT Antibody (N-term D121) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

KIT Antibody (N-term D121) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of KIT Antibody (N-term D121) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

KIT

UniProt

P10721

RefSeq (Protein):NP_001087241.1, NP_000213.1