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KLF2 Antibody
SKU: orb1258988
Featured
Description
Research Area
,Cancer,Transcription
Images & Validation
−Item 1 of 5
| Tested Applications | IF, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human KLF2 (NP_057354.1). |
| Target | KLF2 |
| Molecular Weight | Observed: 37kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−KLF2, Kruppel-like factor 2 (lung), LKLF, Kruppel-like factor LKLF, lung Kruppel-like zinc finger transcription factor
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Western blot analysis of extracts of various cell lines, using KLF2 antibody (orb1258988) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90S.
Western blot analysis of extracts of various cell lines, using KLF2 antibody (orb1258988) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunofluorescence analysis of C6 cells using KLF2 antibody (orb1258988) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using KLF2 antibody (orb1258988) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using KLF2 antibody (orb1258988) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
KLF2
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
KLF2 Antibody (orb1258988)
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