KRAS Antibody (C-term)

SKU: orb527166

Description

Rabbit polyclonal antibody to KRAS

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Cardiovascular Disease,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Primary Antibodies;Cancer;Signal Transduction;Catalog Updated;B cell receptor signaling;T cell receptor signaling;Chemokine signaling;Inflammation mediated by chemokine and cytokine signaling

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution Range: WB: 1:2000, IF: 1:10-50, FC: 1:10-50
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB32708
• Immunogen: Synthetic Peptide
• Target: KRAS
• Molecular Weight: 21656
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase KRas, N-terminally processed, KRAS, KRAS2, RASK2

KRAS Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-KRAS Antibody (C-term) at 1:2000 dilution + HT-29 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of KRAS Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

All lanes: Anti-KRAS Antibody (C-term) at 1:500 dilution. Lane 1: Hela whole cell lysate. Lane 2: 293T/17 whole cell lysate. Lane 3: C2C12 whole cell lysate. Lane 4: C6 whole cell lysate. Lane 5: HT-29 whole cell lysate. Lane 6: PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-KRAS Antibody (C-term) at 1:2000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: C2C12 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: HT-29 whole cell lysate. Lane 5: K562 whole cell lysate. Lane 5: PC-12 whole cell lysate. Lane 5: Ramos whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

KRAS

UniProt

P01116

RefSeq (Protein):NP_004976.2, NP_203524.1