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Description
Research Area
,Product Categories/Antibodies,Adhesion & Migration
Images & Validation
−Item 1 of 5
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100-1:200 |
| Reactivity | Human, Mouse, Rabbit |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / Extracellular domain corresponding to Human L selectin / CD62L |
| Target | L-selectin | CD62L |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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All lanes: Anti L-selectin/CD62L antibody at 1/500 dilution. Lysates at 50 μg per lane, This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294260 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling L-selectin/CD62L with orb1294260 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling L-selectin/CD62L with orb1294260 at 1/100.
Anti L-selectin/CD62L antibody at 1/500 dilution. Lysates at 50 μg per lane, This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294260 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294260 Anti-L-selectin/CD62 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Quick Database Links
Gene Symbol
L-selectin | CD62L
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
L-selectin | CD62L (orb1294260)
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