Lamin A / C Antibody

SKU: orb1258730

Description

Lamin A / C Antibody

Research Area

,Apoptosis,Cell Cycle,Signal Transduction,Stem Cell

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:200 - 1:1000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide of human Lamin A / C
• Target: LMNA
• Molecular Weight: Observed: 74kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LMNA, 70 kDa lamin, CDCD1, CMD1A, EMD2, FPLD, IDC, FPL, FPLD2, Lamin A/C, Lamin, LFP, LMNC, LMN1, LMNL1, HGPS, Prelamin-A/C, PRO1, Lamin A, Lamin A/C-like 1, LGMD1B, CDDC, CMT2B1, LDP1

Western blot analysis of extracts of various cell lines, using Lamin A/C antibody (orb1258730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Immunohistochemistry of paraffin-embedded human mammary cancer using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human uterine cancer using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spinal cord using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of NIH-3T3 cells using BLOC1S3 antibody (orb1254718) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using Lamin A/C antibody (orb1258730) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using Lamin A/C antibody (orb1258730) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

LMNA

UniProt

P02545