Lamin B1 Antibody, KO Validated

SKU: orb1274472

Description

Lamin B1 Antibody, KO Validated

Research Area

,Apoptosis,Cell Cycle,Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 397-586 of human Lamin B1 (NP_005564.1).
• Target: LMNB1
• Molecular Weight: Observed: 70kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LMNB1, lamin B1, ADLD, LMN, LMN2, LMNB, MGC111419, OTTHUMP00000159218, lamin-B1

Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (orb1274472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts from normal (control) and Lamin B1 knockout (KO) HeLa cells, using Lamin B1 antibody (orb1274472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat spinal cord using Lamin B1 antibody (orb1274472) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using Lamin B1 antibody (orb1274472) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using Lamin B1 antibody (orb1274472) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Lamin B1 antibody (orb1274472) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using Lamin B1 antibody (orb1274472) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using Lamin B1 Polyclonal Antibody (orb1274472) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using Lamin B1 Polyclonal Antibody (orb1274472) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

LMNB1

UniProt

P20700