LAMP1 Antibody

SKU: orb306476

Description

Mouse monoclonal antibody to LAMP1

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Infectious Diseases,Crown Antibodies;Primary Antibodies;Stem Cells;Host cell receptor for virus entry;Viral entry into host cell;Host-virus interaction;Lysosome

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF/ICC: 1:25, IHC-P: 1:25, WB: 1:2000
• Reactivity: Human, Monkey

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: 1483CT517.45.23
• Target: LAMP1
• Molecular Weight: 44882
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Lysosome-associated membrane glycoprotein 1 antibody, anti LAMP-1 antibody, anti Lysosome-associated membrane protein 1 antibody, anti CD107 antigen-like family member A antibody, anti CD107a antibody, anti LAMP1 antibody

All lanes: Anti-LAMP1 Antibody at 1:1000 dilution (upper) Lane 1: HT1080 Lane 2: HT1080-Knockout Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated (ASP1613) at 1/8000 dilution. Predicted band size: 90-120 kDa

All lanes: Anti-LAMP1 Antibody at 1:2000 dilution. Lane 1: A431 whole cell lysates. Lane 2: Hela whole cell lysates. Lane 3: Jurkat whole cell lysates. Lane 4: HT-1080 whole cell lysates. Lane 5: COS-7 whole cell lysates. Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining LAMP1 in human kidney sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling LAMP1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

Quick Database Links

Gene Symbol

LAMP1

UniProt

P11279