LC3 Antibody (APG8B) (C-term)

SKU: orb33329

Description

Purified rabbit polyclonal antibody.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Cardiovascular Disease,Neuroscience,Signaling Pathways,Signaling Pathways/Autophagic,Epigenetics,Primary Antibodies;Antibody Collections;Cancer;Cardiovascular;Metabolism;Neuroscience;Signal Transduction;Autophagy;Autophagy

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:50-100
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB7485
• Immunogen: Synthetic Peptide
• Target: MAP1LC3B
• Molecular Weight: 14688
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-MAP1LC3B antibody, anti-LC3B antibody, anti-MAP1A/1BLC3 antibody, anti-MAP1LC3B antibody

Western blot analysis of anti-LC3 (APG8b) Pab in 293 cell line lysates transiently transfected with the LC3 (APG8b) gene (2 ug/lane). LC3 (APG8b) (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis tissue reacted with Autophagy LC3 antibody (APG8b) (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

MAP1LC3B

UniProt

Q9GZQ8

RefSeq (Protein):NP_073729.1