LDHA Antibody (C-term)

SKU: orb1787968

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Primary Antibodies;Cancer;Metabolism;Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: 204-232 aa
• Target: This LDHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-232 amino acids from the C-terminal region of human LDHA.
• Molecular Weight: 36689 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59, LDHA

Confocal immunofluorescent analysis of LDHA Antibody (C-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

LDHA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of LDHA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-LDHA Antibody (C-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: MDA-MB-231 whole cell lysate. Lane 3: human placenta lysate. Lane 4: PC-3 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

This LDHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-232 amino acids from the C-terminal region of human LDHA.

UniProt

P00338