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Description
Research Area
DNA Binding Proteins, DNA and RNA, Developmental Biology, Nuclear Signaling Pathway, Transcription Factors, DNA and RNA Research, Wnt signaling pathway
Images & Validation
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Item 1 of 3
| Tested Applications | FC, WB |
|---|---|
| Dilution Range | WB=1:500-2000, Flow-Cyt=1ug/test |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Canine, Gallus, Porcine, Rabbit, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human LEF-1 (331-399/399aa) |
| Target | LEF1 |
| Molecular Weight | 44 kDa |
| Purification | affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−lymphoid enhancing factor-1; Transcription factor T cell specific 1 alpha; DKFZp586H0919; LEF 1; LEF1; Lymphoid enhancer binding factor 1; T cell specific transcription factor 1 alpha; TCF 1 alpha; TCF1 alpha; TCF1alpha; TCF1-alpha; LEF1_HUMAN; Lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha.
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Quick Database Links
Gene Symbol
LEF1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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Usually dispatched within 5-10 working days