Leptin Receptor Antibody / LEPR

SKU: orb1151672

Description

Mouse monoclonal antibody to Leptin Receptor

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Epigenetics

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Leptin Receptor antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: LEPR/4301
• Immunogen: A portion of amino acids 335-425 was used as the immunogen for the Leptin Receptor antibody.
• Purity: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot the Leptin Receptor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This Leptin Receptor antibody is available for research use only.
• Disclaimer: For research use only

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Leptin Receptor antibody (clone LEPR/4301). These results demonstrate the foremost specificity of the LEPR/4301 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC staining of human liver tissue with Leptin Receptor antibody (clone LEPR/4301). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Leptin Receptor antibody (clone LEPR/4301) as confirmation of integrity and purity.

Quick Database Links

UniProt

P48357