Lineage Cocktail FITC

SKU: orb1821016

Description

The Lineage Cocktail FITC is designed to stain and gate-out human T lymphocytes (CD3+), B lymphocytes (CD19+CD20+), NK cells (CD16+CD56+), non-differentiated monocytes (CD14+), neutrophils (CD16+), and eosinophils (CD14+dim). Peripheral blood dendritic cells and basophils are Lineage negative and do not stain using this reagent. Whenever applicable, the lineage negative cells can be simultaneously stained by other monoclonal antibodies conjugated to appropriate fluorochromes. For example, dendritic cells can be distinguished by positive staining with anti HLA-DR, and basophils by positive staining with anti CD203c.

Key Properties

• Conjugation: FITC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Flow cytometry multicolor surface staining of human peripheral whole blood stained using Lineage Cocktail FITC (20 μl cocktail / 100 μl of peripheral whole blood) and anti-human CD203c (NP4D6) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human target population cells (red-filled) from basophils (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using Lineage Cocktail FITC (20 μl cocktail / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using Lineage Cocktail FITC (20 μl reagent / 100 μl of peripheral whole blood).