Luciferase Antibody Biotin Conjugated

SKU: orb344289

Description

Luciferase antibody (Biotin)

Research Area

Product Categories/Products/Antibodies,Immunogen Type: Native Protein

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:80,000 - 1:310,000, WB: 1:8,000 - 1:31,000
• Application Notes:
  Anti-Luciferase Peroxidase conjugated antibody is produced from Photinus pyralis (Firefly) which produces a green light with a wavelength of 562 nm. Anti-Luciferase Antibody has been tested by ELISA and western blot. Expect a band at approximately 60.7 kDa in size corresponding to Luciferase by western blotting in the appropriate cell lysate or extract. Anti-Luciferase has been assayed against 1.0 ug of Luciferase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:80,000 to 1:310,000 of the reconstitution concentration is suggested for this product.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Luciferase [Photinus pyralis (Firefly)]
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)]. No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 10.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Luciferase Antibody biotin conjugation, Luciferase firefly Antibody biotin conjugation, goat anti Luciferase Antibody biotin conjugation