LYN Antibody (N-term)

SKU: orb1428534

Description

Rabbit polyclonal antibody to LYN.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Immunology,Epigenetics,Infectious Diseases,Primary Antibodies;Signal Transduction;Anti-Kinome Antibodies;Viral process;Host-virus interaction;Immunity;Inflammatory response;Innate immunity;B cell receptor signaling;Fc gamma R-mediated phagocytosis;Chemokine signaling

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB03080
• Immunogen: Synthetic Peptide
• Target: LYN
• Molecular Weight: 58574
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tyrosine-protein kinase Lyn, Lck/Yes-related novel protein tyrosine kinase, V-yes-1 Yamaguchi sarcoma viral related oncogene homolog, p53Lyn, p56Lyn, LYN, JTK8

Anti-LYN Antibody (E46) at 1:1000 dilution + Raji whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 59 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

LYN Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of anti-LYN Antibody (N-term) in HL60 cell line lysates (35 ug/lane). LYN (arrow) was detected using the purified Pab.

Quick Database Links

Gene Symbol

LYN

UniProt

P07948

RefSeq (Protein):NP_001104567.1, NP_002341.1