MARCKS (phospho Ser152 / 156) Antibody

SKU: orb1273522

Description

MARCKS (phospho Ser152 / 156) Antibody

Research Area

,Signal Transduction,Phospho-Specific,

Images & Validation

• Tested Applications: WB
• Reactivity: Frog, Human, Mouse, Rat
• Application Notes:
  Immunolabeling of the 87kDa band in rat brain homogenate is blocked by the Ser152/156 phosphopeptide but not by the non-phosphopeptide. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB) and Western Blot (WB). Suitability for Immunohistochemistry (IHC) not yet determined. Human, mouse, rat, and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

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Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: MARCKS (Ser152/156) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser152 and phospho-Ser156 of rat MARCKS.
• Target: Marcks
• Molecular Weight: 87
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Marcks

UniProt

P30009

RefSeq:P30009