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Description
Research Area
Images & Validation
−| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Porcine, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | 286-314 aa |
| Target | This MDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-314 amino acids from the C-terminal region of human MDH1. |
| Molecular Weight | 36426 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−MDH1 Antibody (C-term) [orb1931348]
FC, IHC-P, WB
Mouse, Porcine, Rat
Human
Rabbit
Polyclonal
Unconjugated
400 μlMDH1 Antibody (C-term) [orb34311]
FC, IHC-P, WB
Mouse, Porcine, Rat
Human
Rabbit
Polyclonal
Unconjugated
80 μl

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MDH1 Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of lysates from HL-60, THP-1 cell line (from left to right), using MDH1 Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_001186041.1, NP_001186040.1, NP_005908.1 |
|---|
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Protocol Information
MDH1 Antibody (C-term) (orb1788249)
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