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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, ICC, IF, IHC, WB |
|---|---|
| Dilution Range | WB (1:1000); IHC (1:50) |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide from the mid-protein of Human MDM2 (aa. 100-200) |
| Target | MDM2 |
| Molecular Weight | 50 kDa |
| Conjugation | PerCP |
Storage & Handling
−| Storage | Conjugated antibodies should be stored according to the product label |
|---|---|
| Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Immunohistochemistry analysis using Rabbit Anti-MDM2 Polyclonal Antibody. Tissue: Stomach Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Western blot analysis of Mouse, Rat Brain showing detection of ~40 kDa MDM2 protein using Rabbit Anti-MDM2 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Lane 3: Rat Brain. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~40 kDa. Other Band (s): Isoform 2 or 3.
Quick Database Links
UniProt Details
−NCBI Gene Details
−NCBI Reference Sequences
−| Protein | NP_001138811.1 |
|---|
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Protocol Information
MDM2 Antibody: PerCP (orb535763)
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