MICA Antibody (Center)

SKU: orb1788025

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Crown Antibodies;Primary Antibodies;Host-virus interaction;Immunity;MHC class l-like antigen recognition

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: IF: 1:25, WB: 1:1000, WB: 1:2000, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: 68-97 aa
• Target: This MICA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the Central region of human MICA.
• Molecular Weight: 42915 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MHC class I polypeptide-related sequence A, MIC-A, MICA {ECO:0000312|EMBL:CAI419071}

Immunohistochemical analysis of paraffin-embedded H. hepatic carcinoma section using MICA Antibody (Center). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. colorectal carcinoma section using MICA Antibody (Center). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Anti-MICA Antibody (Center) at 1:1000 dilution + Hela whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-MICA Antibody (Center) at 1:1000 dilution. Lane 1: A431 whole cell lysates. Lane 2: Daudi whole cell lysates. Lane 3: Jurkat whole cell lysates. Lane 4: MCF-7 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining MICA in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes: Anti-MICA Antibody (Center) at 1:2000 dilution. Lane 1: Jurkat whole cell lysates. Lane 2: Daudi whole cell lysates. Lane 3: A431 whole cell lysates. Lane 4: MCF-7 whole cell lysates. Lane 5: U-87MG whole cell lysates. Lane 6: THP-1 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling MICA at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on Hela cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

Quick Database Links

Gene Symbol

This MICA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the Central region of human MICA.

UniProt

Q29983

RefSeq (Protein):NP_000238.1