Microphthalmia Transcription Factor (MITF) Antibody

SKU: orb2320450

Description

Microphthalmia Transcription Factor (MITF) Antibody

Research Area

,B Cell Markers|Cancer|Cardiovascular|Mast Cell Marker|Stem Cell Differentiation

Images & Validation

• Tested Applications: IHC
• Dilution Range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: Jurkat, A-431, HeLa or 501 Mel human melanoma cells. Melanoma.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: D5 + MITF/915
• Immunogen: NH2 terminus fragment of human Mi protein (D5); Recombinant human MiTF protein (MITF/915)
• Target: MITF
• Source/Expression System: HEK293 cells
• Molecular Weight: 52-56kDa (doublet)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BHLHE32; Class E basic helix-loop-helix protein 32 (bHLHe32); CMM8; Mi; Microphthalmia-associated transcription factor; MITF; WS2; WS2A

Quick Database Links

Gene Symbol

MITF

UniProt

O75030

NCBI Gene ID

4286