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MLKL monoclonal antibody (M02), clone 3B2
Description
Images & Validation
−| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2a Kappa |
| Clone No. | 3B2 |
| Immunogen | MLKL (NP_689862, 371 a.a. ~ 471 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Protein Sequence | VKSTAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | In 1x PBS, pH 7.4 |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |

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Detection limit for recombinant GST tagged MLKL is 0.3 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to MLKL on HeLa cell. [antibody concentration 10 ug/ml]

Immunoperoxidase of monoclonal antibody to MLKL on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]

Western Blot detection against Immunogen (36.85 KDa).
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Protocol Information
MLKL monoclonal antibody (M02), clone 3B2 (orb2290611)
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