MMP14 / MT1-MMP

SKU: orb1294372

Description

Rabbit polyclonal antibody to MMP14

Research Area

,Product Categories/Antibodies,MMP remodeling

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:500 Immunofluorescence 1:150-1:300 Immunohistochemistry (Paraffin) 1:100-1:200
• Reactivity: Human, Mouse, Rabbit

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human MMP14 / MT1-MMP
• Target: MMP14 / MT1-MMP
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

All lanes: Anti-MMP14/MT1-MMP antibody - Loading Control at 1/500 dilution, Lysates/proteins at 30 μg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with orb1294372 overnight at 4°C.

All lanes: Anti-MMP14/MT1-MMP antibody - Loading Control at 1/500 dilution, Lysates/proteins at 30 μg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with orb1294372 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling MMP14/MT1-MMP with orb1294372 at 1/100.

Customer feedback Image. Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling MMP14/MT1-MMP with orb1294372 at 1/100.

Customer feedback Image. Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling MMP14/MT1-MMP with orb1294372 at 1/100.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294372 anti-MMP14/MT1-MMP antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294372 anti-MMP14/MT1-MMP antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Quick Database Links

Gene Symbol

MMP14 / MT1-MMP